Composite

Part:BBa_K3778039

Designed by: Yun Tsai   Group: iGEM21_CCU_Taiwan   (2021-09-06)


Thrombin releasable TAT48-57-6xHis-Cathepsin S releasable DPK-060

The synthetic protein from this composite part is responsible for intracellular bacterial clearance. The CBD in synthetic protein anchors on collagen, and releases TAT48-57-Cathepsin S releasable DPK-060 as Thrombin appearance. The released TAT48-57-Cathepsin S releasable DPK-060 will penetrate into cells and release DPK-060 in by phagolysosomal Cathepsin S to clear bacteria in macrophage.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 761
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 761
    Illegal NheI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 761
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 761
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None